英语翻译Materials and methodsAnimalsMale Wistar rats (250–340 g) were obtained from theZentrales Tierlaboratorium (Universita¨tsklinikum Essen).Animals were kept under standard conditions with freeaccess to food and water.All animals received

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英语翻译Materials and methodsAnimalsMale Wistar rats (250–340 g) were obtained from theZentrales Tierlaboratorium (Universita¨tsklinikum Essen).Animals were kept under standard conditions with freeaccess to food and water.All animals received

英语翻译Materials and methodsAnimalsMale Wistar rats (250–340 g) were obtained from theZentrales Tierlaboratorium (Universita¨tsklinikum Essen).Animals were kept under standard conditions with freeaccess to food and water.All animals received
英语翻译
Materials and methods
Animals
Male Wistar rats (250–340 g) were obtained from the
Zentrales Tierlaboratorium (Universita¨tsklinikum Essen).
Animals were kept under standard conditions with free
access to food and water.All animals received humane care
in compliance with the institutional guidelines.
Chemicals
RPMI 1640 medium,Leibovitz L-15 medium,penicillin
and streptomycin were obtained from Gibco (Eggenstein,
Germany).Dimethyl sulfoxide (Me2SO),Triton X-100,
ascorbic acid and 8-hydroxyquinoline were purchased from
Merck (Darmstadt,Germany).Fetal calf serum,ferrous
ammonium sulfate,ferric chloride,ionomycin,glutathione,
chelex-100 and EGTA were from Sigma–Aldrich (Taufkirchen,
Germany),and fibronectin was from Roche Molecular
Biochemicals (Mannheim,Germany).Deferoxamine
mesylate (Desferal) was from Novartis Pharma (Nuremberg,
Germany).University of Wisconsin (UW) solution,
obtained from Bristol–Myers Squibb (Princeton,USA),
was supplemented with 200,000 U/l benzylpenicillin (Biochrom,
Berlin,Germany),40 IU/l insulin (Merck),and
16 mg/l dexamethasone (Serva,Heidelberg,Germany).
1,2-Bis(2-aminophenoxy)ethane-N,N,N0,N0-tetraacetic acidacetoxymethyl
ester (BAPTA-AM),Quin-2-acetoxymethyl
ester (Quin-2-AM),Quin-2,free acid,and the fluorescent
dyes Oregon Green\3 488 BAPTA-1-AM (OG BAPTAAM)
and Oregon Green\3 488 BAPTA-1,hexapotassium
salt,were purchased from Molecular Probes Europe BV
(Leiden,the Netherlands).Ruthenium Red and Bax-Inhibiting
Peptide V5 were obtained from Calbiochem (Darmstadt,
Germany)
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英语翻译Materials and methodsAnimalsMale Wistar rats (250–340 g) were obtained from theZentrales Tierlaboratorium (Universita¨tsklinikum Essen).Animals were kept under standard conditions with freeaccess to food and water.All animals received
材料和方法
动物,
雄性老鼠(250-340 g)外提取了
Zentrales（是不是centrales） Tierlaboratorium(Universita”tsklinikum埃森).
动物被保存在标准条件下与自由
获得食物和水.所有的动物都收到的人文关怀
按照这个制度的指导方针.
化学
RPMI 1640介质,Leibovitz L-15介质、青霉素
从Gibco链霉素、(Eggenstein,
德国).二甲基亚砜(Me2SO)、海神X-100,
抗坏血酸和8 -羟基喹啉从
默克(达姆施塔特,德国).胎儿血清、黑色牛犊
硫酸铵,氯化铁、ionomycin,谷胱甘肽,
西格玛奥德里奇来自chelex-100和EGTA(Taufkirchen,
德国)、纤连蛋白是从罗氏分子
生化药品(在德国的曼海姆).尿
(Desferal)是可以从诺华制药(纽伦堡,
德国).威斯康辛大学(刚解),
从百时美施贵宝(北京),
20你是补充benzylpenicillin(Biochrom信用证,
德国柏林国际单位/ 40),胰岛素(默克),以及16毫克/升)地塞米松(Serva德国海德堡大学).
1、2-Bis(2-aminophenoxy)ethane-N、N、没有,N0-tetraacetic acidacetoxymethyl
酯(BAPTA-AM),Quin-2-acetoxymethyl酯(Quin-2-AM),Quin-2、自由酸、荧光dyes Oregon Green\3 488 BAPTA-1-AM (OG BAPTAAM)and Oregon Green\3 488 BAPTA-1,hexapotassium 盐,从分子探讨欧洲
(荷兰)、荷兰).钌红色和Bax-Inhibiting
从Calbiochem 8:28胜肽(达姆施塔特,
德国).

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